FastQCFastQC Report
Sun 19 Mar 2023
SRR4895951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1438110
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG168591.172302535967346No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC115530.8033460583682751No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA86280.5999541064313578No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55460.3856450480144078No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC48620.3380826223306979No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA31680.22028912948244572No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC29930.20812038022126264No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT23890.16612081134266501No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT22460.15617720480352687No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG19890.1383065273171037No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT17870.12426031388419524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA16680.11598556438659074No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC16270.1131346002739707No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA15360.10680685065815551No Hit
AGGATCACAGGCTCGGGTCGCTGCAGACGCTTTCAGTGGGTTGACGGCAG14720.10235656521406568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT333700.0139.664031
GGTATCA333900.0139.488728
GTATCAA336600.0138.412619
GTGGTAT337550.0138.087046
TGGTATC337950.0137.90237
CAGTGGT340100.0137.05174
AGCAGTG341100.0136.549622
AGTGGTA343400.0135.713685
GCAGTGG346700.0134.344043
CAACGCA334150.028.45862610-14
TCAACGC334900.028.39919310-14
AGAGTAC333100.028.37109615-19
GTACATG331700.028.34324320-24
ATCAACG335450.028.30970410-14
TACATGG331450.028.2951120-24
CAGAGTA334500.028.26096215-19
CGCAGAG335800.028.2287415-19
AGTACAT333550.028.16877220-24
GCAGAGT338300.027.88392815-19
AACGCAG342750.027.75296810-14
Produced by FastQC (version 0.11.5)