FastQCFastQC Report
Sun 19 Mar 2023
SRR4895950_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895950_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979942
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC981650.0142.294711
ATCAGCA981800.0142.272982
TCAGCAG322550.0141.731233
TCAGCAT303250.0141.632193
TCAGCAC122050.0140.212923
TCAGCAA241950.0140.133263
CAGCATA82050.0139.855964
CAGCAAT82650.0138.581954
CAGCATG101600.0138.550174
CAGCAGT103700.0138.424944
CAGCATT94050.0137.698684
AGCATAG29350.0137.691975
CAGCAGA81750.0136.184274
CAGCAAG79100.0135.971044
CAGCAGG116800.0135.043054
AGCATGA25500.0134.722635
AGCATTG33950.0133.941315
AGCATGT34650.0133.90955
CAGCACG34250.0133.392414
AGCAGAT27950.0133.113575
Produced by FastQC (version 0.11.5)