FastQCFastQC Report
Sun 19 Mar 2023
SRR4895950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979942
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG138841.4168185464037668No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC87600.893930457108686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC51130.5217655738809032No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30940.3157329719514012No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA28220.2879762271644648No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC25650.26175018521504334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT24350.24848409395658108No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT22280.22736039479887585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA19330.19725657232775004No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG15370.15684601741735735No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT14070.14357992615889512No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA13200.13470184970130886No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC13060.1332731937196283No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACA12610.12868108520708368No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC12360.12612991381122557No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT12140.12388488298287041No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA11510.11745593106530794No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTCTAAGCTGCAGCAAGA11080.11306791626443198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTAAGCTGCAGCAAGAG10970.11194540085025441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT271150.0140.345261
GGTATCA274400.0139.196588
GTGGTAT275750.0138.332346
GTATCAA276250.0138.316519
TGGTATC277850.0137.416387
CAGTGGT278600.0136.89144
AGCAGTG278550.0136.565122
AGTGGTA280450.0136.039725
GCAGTGG281600.0135.239463
CTACGCG304.6152607E-528.799654105-109
CGAGGTA200.00628605628.799652125-129
CAACGCA274250.028.49511510-14
ATCAACG275000.028.3964610-14
AGAGTAC274250.028.37960215-19
GTACATG273800.028.35261720-24
TACATGG273700.028.32088720-24
CGCAGAG275100.028.31285515-19
TCAACGC276200.028.3095810-14
CAGAGTA275150.028.27630815-19
AGTACAT274800.028.2127620-24
Produced by FastQC (version 0.11.5)