FastQCFastQC Report
Sun 19 Mar 2023
SRR4895949_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895949_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441096
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATG1445000.0142.151951
AGGATGA1447300.0141.926032
GGATGAT501250.0141.219253
GGATGAG410850.0140.581343
GGATGAA373600.0140.55763
GGATGAC173650.0140.243183
GATGACC55800.0137.177114
GATGAAT139000.0136.491264
GATGATA139850.0135.253984
GATGACG47550.0135.197174
GATGATG163850.0133.972564
GATGATC58850.0133.943074
GATGAAG115050.0133.930854
GATGAGA115050.0133.868914
GATGACT44000.0133.632744
GATGAGT133350.0133.616044
ATGACGT13700.0132.65735
GATGATT166250.0132.552954
GATGAGC46500.0132.425724
GATGAAC32800.0132.329014
Produced by FastQC (version 0.11.5)