FastQCFastQC Report
Sun 19 Mar 2023
SRR4895949_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895949_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441096
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG199521.3845017958553767No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC133420.9258231235115495No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA58570.40642677517667114No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43600.3025475055096954No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC35710.2477975096731932No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC29060.20165207591999423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG22520.15626995009353992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT22350.15509029238857094No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA22160.1537718514241938No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA16630.11539828019784941No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT15280.10603041018780152No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC15280.10603041018780152No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT14520.10075664633029306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT388600.0140.071721
GGTATCA389100.0139.885048
GTGGTAT391000.0139.20536
TGGTATC392100.0138.869867
GTATCAA392550.0138.747349
AGCAGTG397200.0136.984562
CAGTGGT398300.0136.744344
GCAGTGG401850.0135.417363
AGTGGTA403100.0135.062455
CAACGCA390050.028.44046810-14
ATCAACG389650.028.44009810-14
TCAACGC390200.028.42953310-14
AGAGTAC387200.028.41923515-19
GTACATG385650.028.40042120-24
CAGAGTA388150.028.36081115-19
TACATGG386350.028.28187620-24
AGTACAT388150.028.22863220-24
CGCAGAG392500.028.1602215-19
GCAGAGT391900.028.08943215-19
GAGTACA386250.027.92756820-24
Produced by FastQC (version 0.11.5)