FastQCFastQC Report
Sun 19 Mar 2023
SRR4895948_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895948_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705221
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG140501.9922832700671138No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC99741.4143084224661489No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC37510.5318900032755689No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC29750.42185357497862375No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA28010.3971804583244118No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG21800.30912295578265536No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT20650.2928160108675153No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19760.2801958534984069No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA17450.24744016414712552No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT15220.2158188709638539No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT15180.215251672879849No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT14510.20575110497276738No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTAAGCTGCAGCAAGAG12810.18164518640256033No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC11770.16689803621843366No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTCTAAGCTGCAGCAAGA11580.1642038453194105No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA11300.1602334587313764No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA11280.15994985968937397No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC10550.14959849465628505No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCGAGATTCCCCCAGTAG10440.14803869992527166No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC8930.12662697225408773No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGATTCCCCCAGTAGCGGC8840.12535077656507676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA8140.11542481009499149No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAGAAATCAACCGAGAT7950.11273061919596836No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC7600.10776763596092571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT230550.0140.067861
GGTATCA231950.0139.246468
GTATCAA233700.0138.203749
AGCAGTG234850.0137.441932
GTGGTAT235050.0137.44066
TGGTATC235850.0136.974417
CAGTGGT236400.0136.625274
GCAGTGG237350.0136.085313
AGTGGTA240550.0134.298115
TAGCGAT200.006286385528.799122120-124
CAACGCA231700.028.51324310-14
GTACATG231200.028.42081520-24
TACATGG230950.028.39546420-24
TCAACGC232650.028.39062310-14
ATCAACG232700.028.38452110-14
AGAGTAC232500.028.3160415-19
AGTACAT232150.028.30451220-24
CAGAGTA232750.028.27324915-19
CGCAGAG234000.028.20221715-19
GCAGAGT234600.027.98277915-19
Produced by FastQC (version 0.11.5)