FastQCFastQC Report
Sun 19 Mar 2023
SRR4895947_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895947_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2700101
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGTC2709250.0142.067731
TCTGTCA2709700.0142.044132
CTGTCAG845100.0141.469643
CTGTCAT844400.0141.359013
CTGTCAA705800.0140.994023
TGTCATG278250.0139.526574
TGTCATA235700.0138.708764
TGTCAGA218700.0138.670944
CTGTCAC335750.0138.388383
TGTCAGG291150.0138.26514
TGTCAGT267750.0137.89074
TGTCAAT232650.0137.31044
TGTCAAG235500.0137.252764
GTCATGA76800.0135.774375
GTCAATG80500.0135.554525
TGTCACG90900.0135.021824
GTCATAG81950.0134.98255
TGTCATT251450.0134.073884
GTCATGG99000.0133.765825
TGTCAGC90350.0133.398254
Produced by FastQC (version 0.11.5)