FastQCFastQC Report
Sun 19 Mar 2023
SRR4895947_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895947_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2700101
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG343031.2704339578408363No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC221200.819228614040734No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC169600.6281246516334019No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG107660.39872582544134455No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74580.2762118898515278No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC65140.24125023471344217No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT49000.1814746929837069No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA40340.1494018186727089No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC38710.14336500745712846No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC35980.13325427456232194No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA34100.12629157205600827No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA30620.11340316528900216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT28760.10651453408594715No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTTCTTGCTGCAGCAACG28570.10581085670498994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA745550.0138.985238
AAGCAGT744900.0138.883471
GTGGTAT748800.0138.276216
TGGTATC753750.0137.415887
GTATCAA757700.0136.823069
AGCAGTG759550.0136.157332
AGTGGTA760850.0136.105185
CAGTGGT761700.0135.991124
GCAGTGG773750.0133.779533
CAACGCA743450.028.54205910-14
TCAACGC743950.028.50932710-14
ATCAACG746250.028.38672310-14
GTACATG742300.028.3282720-24
TACATGG742500.028.3206420-24
AGAGTAC745650.028.31911815-19
CAGAGTA749000.028.21168115-19
AGTACAT747100.028.15397520-24
CGCAGAG753350.028.07554215-19
AACGCAG762750.027.80663910-14
GAGTACA742800.027.74313420-24
Produced by FastQC (version 0.11.5)