FastQCFastQC Report
Sun 19 Mar 2023
SRR4895945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008681
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG86810.8606288806867584No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC63210.6266599648451789No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC43000.42629929581304693No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG29640.29384909599764447No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25360.25141744515857833No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC18120.1796405404681956No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT15830.15693762448187287No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC14730.1460322936587484No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA14110.139885652649351No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCTGTGGCTCACG11080.10984642320019906No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA10760.10667396332438103No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT10590.10498859401535272No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC10320.10231183099513126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA266050.0139.182148
AAGCAGT267000.0138.721311
GTGGTAT267850.0138.13936
TGGTATC270100.0137.095187
AGTGGTA272000.0136.08465
GTATCAA272550.0135.994899
AGCAGTG272200.0135.886032
CAGTGGT273100.0135.51014
GCAGTGG275500.0134.232223
CAACGCA265650.028.5288210-14
TCAACGC266250.028.45371210-14
ATCAACG267400.028.2990310-14
AGAGTAC266200.028.24808715-19
GTACATG266100.028.19917920-24
TACATGG265950.028.18259620-24
CAGAGTA267550.028.14322915-19
AGTACAT266650.028.0654120-24
CGCAGAG269650.027.94007515-19
AACGCAG272300.027.8268110-14
GAGTACA265600.027.6179320-24
Produced by FastQC (version 0.11.5)