FastQCFastQC Report
Sun 19 Mar 2023
SRR4895944_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895944_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227703
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA1230350.0142.248182
GGTGCGA1231250.0142.144211
TGCGAAG409050.0141.875643
TGCGAAT393900.0141.759033
GCGAATG135300.0141.290884
GCGAATA104050.0140.568634
GCGAAGT128750.0140.506854
TGCGAAA290400.0140.198943
TGCGAAC145850.0140.062823
GCGAATT107600.0139.971744
GCGAAAT98800.0139.52524
CGAATAG34450.0139.037895
GCGAAGA99550.0138.760444
GCGAAGG146250.0138.363864
CGAATGA34000.0138.362415
CGAAGTA36650.0138.276635
CGAATAT34550.0138.016575
CGAATGT45800.0137.886385
CGAATGG49300.0137.494955
CGAAAGT36500.0137.477925
Produced by FastQC (version 0.11.5)