FastQCFastQC Report
Sun 19 Mar 2023
SRR4895944_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895944_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227703
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG164391.33900462896971No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC105070.8558258797119498No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC87110.709536426969715No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG54690.44546604512654936No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36290.2955926636979791No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC26120.21275503928881823No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA18790.15305004549145845No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT17490.1424611652818312No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA15630.12731092128959529No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT15620.12722946836490584No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTTCTTGCTGCAGCAACG13860.11289375361956434No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC12980.10572589624689359No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT12590.10254923218400543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA305950.0139.57338
AAGCAGT306950.0139.16961
GTGGTAT307450.0138.82216
TGGTATC310200.0137.661037
GTATCAA310850.0137.488989
CAGTGGT312950.0136.451354
AGCAGTG314050.0135.97742
GCAGTGG315500.0135.375293
AGTGGTA316050.0134.976275
TCAACGC307050.028.44776710-14
CAACGCA306850.028.44284610-14
ATCAACG306550.028.437810-14
AGAGTAC305850.028.4134315-19
CAGAGTA305950.028.39943515-19
GTACATG304950.028.34215420-24
TACATGG306100.028.17451720-24
AGTACAT307150.028.15790420-24
CGCAGAG311450.027.96727415-19
GAGTACA305550.027.76808520-24
GCAGAGT315450.027.55329715-19
Produced by FastQC (version 0.11.5)