FastQCFastQC Report
Sun 19 Mar 2023
SRR4895943_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895943_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800567
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAC1809450.0141.845571
GAGAACA1809700.0141.825962
AGAACAT580050.0140.371813
AGAACAG584350.0139.363273
GAACATG193000.0139.223074
GAACAGT185850.0139.133564
GAACAAT152200.0137.442354
GAACATA158200.0137.140374
GAACATT172500.0136.05984
GAACAGA149300.0135.529164
AGAACAA459750.0135.353473
AGAACAC233850.0135.018573
GAACATC70000.0134.09574
GAACAAG158000.0133.118774
GAACAGG204750.0132.591054
GAACACT55100.0132.198474
GAACACG64050.0130.644
AACATGG70800.0129.359125
AACATAG57550.0128.675955
AACAGTG64150.0128.325355
Produced by FastQC (version 0.11.5)