FastQCFastQC Report
Sun 19 Mar 2023
SRR4895943_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895943_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800567
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG137480.7635372635397627No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC110760.6151395643705566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69950.38848873715890603No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC50540.2806893606291796No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG39780.22093040692181964No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA28170.1564507180238225No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC26990.14989722681799678No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGATCCCAAGGCCCAACTC22210.12335003362829598No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTAAGCTGCAGCAAGAG21670.12035097833071472No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA19870.11035412733877718No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTCTAAGCTGCAGCAAGA18630.10346740776655354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA379300.0139.13868
GTGGTAT380700.0138.570186
AAGCAGT380450.0138.50661
TGGTATC382350.0137.991037
GTATCAA384450.0137.293449
CAGTGGT386800.0136.329034
AGTGGTA388250.0135.838425
AGCAGTG389050.0135.537452
GCAGTGG393850.0133.90393
CAACGCA377900.028.51381310-14
ATCAACG378550.028.46485510-14
TCAACGC379700.028.38243710-14
GTACATG376450.028.35635620-24
AGAGTAC378550.028.34312815-19
AGTACAT377900.028.24755320-24
TACATGG378200.028.20991520-24
CAGAGTA381000.028.16464815-19
CGCAGAG382050.028.12870215-19
GCAGAGT385650.027.81758315-19
GAGTACA378050.027.7373620-24
Produced by FastQC (version 0.11.5)