FastQCFastQC Report
Sun 19 Mar 2023
SRR4895942_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895942_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500975
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTA501850.0142.292222
GCTCGGT502450.0142.12231
TCGGTAT154900.0141.995713
TCGGTAG167300.0141.610613
TCGGTAC58200.0141.568163
TCGGTAA123600.0141.452213
CGGTATA41350.0141.168934
CGGTAAT41000.0140.98334
CGGTATG53350.0140.81114
CGGTAGA39250.0140.731964
CGGTAAG43950.0140.277474
CGGTAGG60100.0140.057424
CGGTATC15400.0139.770954
CGGTAGT51950.0139.255134
CGGTATT46750.0138.888874
CGGTACG17250.0136.350114
CGGTAAA31050.0133.595584
CGGTACA11700.0133.410194
GGTACGA3550.0132.509285
CGGTAGC19050.0132.07194
Produced by FastQC (version 0.11.5)