FastQCFastQC Report
Sun 19 Mar 2023
SRR4895942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500975
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG96931.934827087179999No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC74241.4819102749638207No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC31050.6197914067568242No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG23110.46130046409501474No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC19080.3808573282099905No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA15710.31358850242028047No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13350.2664803632915814No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT12350.24651928738959028No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTAAGCTGCAGCAAGAG11140.22236638554818106No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA9190.18344228753929837No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC8290.16547731922750636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTCTAAGCTGCAGCAAGA8110.16188432556514795No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA7220.14411896801237586No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC6940.13852986675981838No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT6110.12196217376116572No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCGAACGGGGAGCAGCCC5620.11218124656919008No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC5280.1053944807625131No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC5240.10459603772643347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT147950.0139.672971
GGTATCA149450.0138.305488
GTGGTAT150000.0137.798366
GTATCAA150150.0137.660699
CAGTGGT151450.0136.66924
TGGTATC151700.0136.206687
AGCAGTG152350.0135.639082
GCAGTGG153950.0134.322953
AGTGGTA155450.0133.059845
CAACGCA148950.028.44030410-14
GTACATG148300.028.39132520-24
TACATGG148450.028.33353420-24
ATCAACG149150.028.32493610-14
AGAGTAC149300.028.30612215-19
TCTCGCA2800.028.2865670-74
TCAACGC149750.028.27875510-14
CAGAGTA150050.028.18383215-19
AGTACAT149150.028.16194320-24
CGCAGAG150150.028.10752115-19
GCAGAGT151400.027.86594815-19
Produced by FastQC (version 0.11.5)