FastQCFastQC Report
Sun 19 Mar 2023
SRR4895941_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895941_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78573
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGAG1050.0142.555716
AGGTAGA100.0074475906142.55578
GATACTG100.0074475906142.55577
GATAATG151.311611E-4142.55577
AATCATA100.0074475906142.55578
AGATATG100.0074475906142.55578
GGGCTAG100.0074475906142.55579
CGACGTC202.3053108E-6142.55576
CGAGTAG550.0142.55576
GAGTACT100.0074475906142.55577
GATCCTG100.0074475906142.55577
GAATCTG100.0074475906142.55577
GATCAAT100.0074475906142.55577
CGATTGT800.0142.55576
ATAGAAT100.0074475906142.55578
CGACATG254.0723535E-8142.55576
CGATTGA850.0142.55576
CGATTAG900.0142.55576
TGAGTAG100.0074475906142.55579
GAATAAG202.3053108E-6142.55577
Produced by FastQC (version 0.11.5)