FastQCFastQC Report
Sun 19 Mar 2023
SRR4895941_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895941_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78573
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC37574.781540732821707No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA17452.2208646736156186No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT16062.0439591208175836No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA13001.6545123642981687No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACA8101.0308884731396282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGATTCCCCCAGTAGCGGC7330.9328904331004289No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCGAACGGGGAGCAGCCC6080.7738027057640665No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA5920.7534394766650122No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAGAAATCAACCGAGAT5700.7254400366538124No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTAATGAGGCGAACC5600.7127130184669034No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAACCGAGATTCCCCCAGTA5450.6936224911865398No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCATCTAAGTACCCCGAGGAA5090.6478052257136675No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCGAGATTCCCCCAGTAG4980.6338055057080677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAATCAACCGAGATTCCCC4950.629987400251995No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC4800.6108968729716314No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAACCGGGGGAACTGAAACA4250.540898272943632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTGTGAGGTTAAGCGACTA2960.3767197383325061No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCGACTAAGCGTACACGGT2800.3563565092334517No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCATCTCCTCTAAC2680.3410840874091609No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGAGATTCCCCCAGTAGCGG2610.33217517467832464No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGAGATTCCCCCAGTAGCG2430.3092665419418884No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGTACACGGTGGATGCC2400.3054484364858157No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTAAGCGTACACGGTGGA2310.2939941201175977No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2110.2685400837437797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGGACGTGCTAATCTGCG2040.25963117101294336No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGGTTAATGAGGCGAACCG1990.25326766191948885No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGAGCGAACGGGGAGCAGCC1970.2507222582821071No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGGCAGTCAGAGGCGATGA1970.2507222582821071No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCCTGGAGATTGTC1960.24944955646341616No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1910.24308604736996167No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAATTCTGGACATTAAT1900.24181334555127082No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTCTTGCTTCAACA1880.23926794191388898No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.2367225382765072No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCATCTCCTCTAACT1820.2316317310017436No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCTAAGTACCCCGAGGAAA1820.2316317310017436No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGAGCGAACGGGGAGC1790.22781362554567092No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCCAGAGCCTGAATCAGT1670.2125412037213801No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATTCCCCCAGTAGCGGCGA1630.2074503964466165No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCGTACACGGTGGATGC1620.20617769462792562No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGCGGCGAGCGAACGGGG1540.19599608007839844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAGCGTACACGGTGGATG1460.18581446552887124No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1410.17945095643541675No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAACGGGGAGCAGCCCAGAG1410.17945095643541675No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATGAAGGACGTGCTAATCT1390.17690555279803494No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCGACTAAGCGTACACG1360.17308744734196227No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCT1350.17181474552327133No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCGTCGGTAAGGTGATATG1250.15908772733636237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACTAAGCGTACACGGTG1230.15654232369898055No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGACTAAGCGTACACGG1220.15526962188028967No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCCCCAGTAGCGGCGAG1200.15272421824290786No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1100.1399972000559989No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAGCGTCTGGAAAGGCGC1050.13363369096254438No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTTAATGAGGCGAACCGG1030.1310882873251626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA1010.12854288368778077No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA980.12472477823170808No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCAAAAAAAAAAAAAAA920.1170885673195627No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCGAACGGGGAGGAGCCC920.1170885673195627No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCGGGGGAACTGAAACAT920.1170885673195627No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGAAGCGTCTGGAAAGGC920.1170885673195627No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGAGCTCGCATTT890.11327046186349No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACA890.11327046186349No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCCTGAATCAGTGTGTG850.10817965458872641No Hit
ATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTAT850.10817965458872641No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAGCAGCCCAGAGCCTG840.10690695277003552No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCATCGACAATAAAC840.10690695277003552No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTACAGCAAAAAAAAAAAA830.1056342509513446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGACGTGCTAATCTGCGATA810.10308884731396281No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGTA100.0072264955144.05
AAGCAGT43450.0142.177221
GTATCAA44850.0138.862889
GGTATCA44850.0138.702358
AGCAGTG44600.0138.511222
AGTGGTA44850.0138.060215
CAGTGGT44900.0137.906464
GCAGTGG44850.0137.899673
GTGGTAT46750.0132.449196
TGGTATC47200.0131.338997
ATGGGTA700.028.80000125-29
GGAGCGT255.3609966E-428.80000125-29
TCCTCTA502.6248017E-928.80000140-44
TCGATTT502.6248017E-928.800001135-139
TAACGGT502.6248017E-928.80000185-89
TGTTCGA502.6248017E-928.800001130-134
AGCGCAG700.028.80000145-49
ACCGTAA304.6042212E-528.800001140-144
CGGTATT502.6248017E-928.80000190-94
GAACATG255.3609966E-428.80000120-24
Produced by FastQC (version 0.11.5)