FastQCFastQC Report
Sun 19 Mar 2023
SRR4895940_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895940_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392412
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATCG100.0074622324142.534158
GTAACGT3100.0142.534155
AGTACGA100.0074622324142.534158
CGAGTAA392500.0142.497832
GCGAGTA392700.0142.425261
GAGTAAG140600.0142.077963
GAGTAAT126350.0141.85733
GAGTAAC36200.0141.156053
GAGTAAA91550.0141.132933
GTAATCG4150.0140.816865
AGTAACG11100.0139.323934
AGTAAGG49700.0139.236084
AGTAATG46750.0139.18044
AGTAAGT49100.0138.760334
AGTAAGA34750.0138.637534
GTAAGGC6650.0138.24745
AGTAAAG37750.0137.814484
TAATCGT1500.0137.7836
AGTAAAT32700.0137.739434
AGTAATA33100.0137.151444
Produced by FastQC (version 0.11.5)