FastQCFastQC Report
Sun 19 Mar 2023
SRR4895940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392412
Sequences flagged as poor quality0
Sequence length150
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA65131.659735176294303No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34100.8689846385941306No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA29810.7596607647064819No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC27410.6985005555385666No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA21420.5458548668236445No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG19960.5086490729131626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA19710.5022782177915048No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCAAAAAAAAAAAAAAA17760.4525855478425736No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGGGGCGGGCGCCGG16590.4227699458732149No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAAAAAAAAAAAAAAAAAA12840.32720711904834715No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAAAAAAAAAAAAAAA12770.3254232796142829No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC12170.3101332273223041No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAGCAAAAAAAAAAAAAAA12130.30911389050283883No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGGGGGGATGCGTGCATT11400.2905109935475979No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA10590.26986942295342653No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAAAAAAAAAAAAAAAAAA10030.25559870748091296No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA9240.2354668052964741No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA8840.22527343710182154No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCACACCTTAAAAAAAAAAA8540.21762841095583213No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAAAAAAAAAAAAAAAAA8030.20463186650765014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAATAGTGGGAAGATT7520.19163532205946812No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCGAACGGGGAGCAGCCC7440.18959664842053758No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCCCTGGACTCATCATC7380.18806764319133973No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAAAAAAAAAAAAAAAAA7340.18704830637187445No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA7270.18526446693781026No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCCAAAAAAAAAAAAAAAAA7160.1824612906842808No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAATCAACCGAGATTCCCC6910.17609043556262297No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTAAAAAAAAAAAAAAAAA6850.17456143033342505No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGATTCCCCCAGTAGCGGC6710.1709937514652967No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAGAAATCAACCGAGAT6590.16793574100690092No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACA6400.16309389111444095No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCAAAAAAAAAAAAAAAAA6340.16156488588524306No Hit
AAGCAGTGGTATCAACGCAGAGTACATGAGAGAAAAAAAAAAAAAAAAAA6310.1608003832706441No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCAAATAAAATTGAAAAA6170.15723270440251574No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAAAAAAAAAAAAAAAAA6070.1546843623538526No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACATTCCAAAAAAAAAAAAA5910.15060701507599156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACAAAAAAAAAAAAAAAAA5820.14831350723219472No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAAAAAAAAAAAAAAAAA5230.1332782891450822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA5130.13072994709641905No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTCAAATAAAATTGAAAA5090.12971061027695382No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCCGGCGGCTTTGGTG5090.12971061027695382No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGAAAAAAAAAAAAAAAAA4970.12665259981855806No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT4950.12614293140882543No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCACAAAAAAAAAAAAA4920.12537842879422648No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAACCGAGATTCCCCCAGTA4830.12308492095042965No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT4690.11951724208230124No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGCAAAAAAAAAAAAAAA4490.11442055798497497No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAAAAAAAAAAAAAAAAA4400.11212705014117814No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCACAAAAAAAAAAAAAAAA4400.11212705014117814No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAAAAAAAAAAAAAAAAAA4250.10830453706818344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCGACTAAGCGTACACGGT4220.1075400344535845No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTAATGAGGCGAACC4160.10601102922438663No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGAAAAAAAAAAAAAAAAA4160.10601102922438663No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAAAAAAAAAAAAAAAA4150.1057561950195203No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCAAAAAAAAAAAAAAAAA4120.10499169240492136No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGGATGCGTGCATTTA4100.10448202399518873No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCGAGATTCCCCCAGTAG4060.10346268717572349No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAACCGGGGGAACTGAAACA4010.10218851615139192No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCAAAAAAAAAAAAAAAAA3980.10142401353679295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT338400.0143.08081
AGCAGTG344850.0140.362872
CAGTGGT345300.0140.308064
GGTATCA345750.0140.292048
GTATCAA346350.0140.132149
TGGTATC346550.0139.94747
GTGGTAT346150.0139.94276
GCAGTGG346200.0139.836333
AGTGGTA347000.0139.641425
GTTATCA1004.956746E-850.3995558
GTGTTAT1459.164014E-734.7583126
TGTTATC1501.1945376E-633.59977
AGTGTTA1501.1945376E-633.59975
GCATTGG1453.6506477E-529.7966373
CGAGTCG200.00628505128.799746125-129
ACGCTAC200.00628505128.799746115-119
CCGACAG200.00628505128.799746135-139
CCGAAAT200.00628505128.799746135-139
CAACGCA345700.028.68727910-14
ATCAACG345950.028.63324710-14
Produced by FastQC (version 0.11.5)