FastQCFastQC Report
Sun 19 Mar 2023
SRR4895939_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895939_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1795
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATAT900.0142.42
CACATAG450.0142.42
CACATAA400.0142.42
TATGAGT100.0068628346142.46
ACATATT303.929017E-10142.43
ACATATG252.6442649E-8142.43
ACATATC201.7358288E-6142.43
AGTGTTG100.0068628346142.47
ATAGTGT100.0068628346142.45
ACATAGT151.1094554E-4142.43
ACATAGG100.0068628346142.43
ACATAGC151.1094554E-4142.43
ACATAAT100.0068628346142.43
ACATAAG151.1094554E-4142.43
ACATAAA100.0068628346142.43
CCACATA1750.0142.41
TATTTAG151.1094554E-4142.46
ATATGGT100.0068628346142.45
CATAGTG151.1094554E-4142.44
ATATGAG100.0068628346142.45
Produced by FastQC (version 0.11.5)