FastQCFastQC Report
Sun 19 Mar 2023
SRR4895938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526471
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG176451.1559341775900098No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC134470.8809207642988305No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC49500.32427736917373473No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43910.2876569551599736No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG34740.22758375363829383No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC28370.1858535144133102No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA23860.15630824299970322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA406850.0138.511378
AAGCAGT407700.0138.179111
GTGGTAT409000.0137.677636
TGGTATC411050.0137.11367
GTATCAA411750.0136.880519
CAGTGGT417950.0134.884434
AGCAGTG418400.0134.593732
AGTGGTA419350.0134.296775
GCAGTGG422150.0133.364013
CAACGCA406700.028.42098810-14
TCAACGC406550.028.41730510-14
ATCAACG407250.028.31895610-14
AGAGTAC408850.028.16290515-19
GTACATG407600.028.16057820-24
CAGAGTA409700.028.11853415-19
TACATGG408700.028.02841220-24
CGCAGAG411950.028.01389115-19
AGTACAT411350.027.88285420-24
AACGCAG417550.027.68591910-14
GAGTACA406900.027.55077720-24
Produced by FastQC (version 0.11.5)