FastQCFastQC Report
Sun 19 Mar 2023
SRR4895937_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895937_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332717
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAC2335500.0142.367482
ATAGCGA2335550.0142.364441
AGCGACT680350.0141.97273
AGCGACG707000.0141.762183
GCGACTA173700.0141.307464
AGCGACA556800.0140.976793
GCGACGT220800.0140.827654
GCGACTG216600.0140.597064
AGCGACC406750.0140.593463
GCGACTT211800.0140.553074
GCGACGA176100.0140.069644
GCGACAT183500.0139.897344
CGACGTA62050.0139.20755
CGACGAT61200.0138.928315
CGACTAT58750.0138.171235
GCGACAA154950.0138.168854
GCGACAG174100.0137.585144
CGACTGA53800.0137.504495
GCGACGG241250.0136.984544
CGACATT58300.0136.670585
Produced by FastQC (version 0.11.5)