FastQCFastQC Report
Sun 19 Mar 2023
SRR4895937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332717
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG260651.117366572970489No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC183210.785393170281693No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA110970.47571137004617364No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC80840.34654868121593835No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63540.2723862345925374No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG58830.25219518698581955No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA35140.15063979042464218No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC23440.10048368490476985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA491550.0139.101768
AAGCAGT490850.0139.051961
GTGGTAT492300.0138.787486
TGGTATC495800.0137.880347
GTATCAA496900.0137.57519
CAGTGGT501950.0136.176674
AGCAGTG502250.0135.824072
AGTGGTA507450.0134.643945
GCAGTGG512050.0133.393373
TCAACGC489900.028.46956810-14
ATCAACG490400.028.41998910-14
AGAGTAC489050.028.37772215-19
CAACGCA493100.028.29941410-14
CAGAGTA491500.028.24798615-19
GTACATG489750.028.2054620-24
AGTACAT490700.028.15085220-24
TACATGG490200.028.11200320-24
CGCAGAG498700.027.94987515-19
GAGTACA487550.027.82178120-24
GCAGAGT500850.027.70339415-19
Produced by FastQC (version 0.11.5)