FastQCFastQC Report
Sun 19 Mar 2023
SRR4895936_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895936_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779028
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG80871.038088489759033No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC60240.7732713073214313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC35200.4518451198159758No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22260.285740692247262No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA20540.2636618966198904No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA14740.18921014392293986No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT14540.18664284210580365No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT14160.1817649686532448No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT13810.1772721904732564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC13660.17534671411040423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC12010.1541664741190304No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG10100.1296487417653794No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC8540.10962378759171686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA8540.10962378759171686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA8190.10513100941172847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT283400.0140.63521
GGTATCA286900.0139.251368
GTATCAA288050.0138.77049
GTGGTAT287800.0138.690816
TGGTATC289150.0138.117987
CAGTGGT289700.0137.83094
AGCAGTG289750.0137.478532
AGTGGTA291000.0137.190435
GCAGTGG292600.0136.262513
ATCAACG286800.028.46749110-14
TACATGG284150.028.4238620-24
TCAACGC287500.028.42322210-14
CAACGCA287700.028.40846610-14
AGAGTAC286250.028.39642715-19
CAGAGTA287050.028.35240415-19
GTACATG285400.028.33973120-24
CGCAGAG288900.028.25557915-19
AGTACAT286600.028.22107120-24
GCAGAGT288100.028.20908515-19
ACATGGG287950.027.87373220-24
Produced by FastQC (version 0.11.5)