FastQCFastQC Report
Sun 19 Mar 2023
SRR4895935_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895935_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512589
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATGC513350.0142.305942
AGCCATG513450.0142.278211
CCATGCG173850.0141.995153
CATGCGA41400.0141.495224
CCATGCA114500.0141.221023
CATGCGG59300.0140.965764
CATGCGT54250.0140.557624
CCATGCT131700.0140.471833
ATGCGAG13500.0138.832875
CATGCAG39100.0138.336044
ATGCGAT13450.0138.289295
CCATGCC99100.0138.213383
CATGCTG48600.0138.129034
ATGCGTA14550.0137.140385
ATGCGGT18250.0137.061225
CATGCCG25600.0136.960544
CATGCGC20800.0136.703584
ATGCGAA11500.0136.331185
CATGCTT38650.0136.074654
ATGCGTG19450.0135.200125
Produced by FastQC (version 0.11.5)