FastQCFastQC Report
Sun 19 Mar 2023
SRR4895935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512589
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG56961.111221661018867No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC43100.840829592519543No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA32020.624672008178092No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC20040.39095649731071097No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13950.272147861151917No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGATGCAAATGTTTTGAAA13350.2604425768012969No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC10900.21264599903626494No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA10430.2034768596282792No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA10280.20055053854062416No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT9050.176554705621853No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT8420.1642641570537019No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG7920.15450975342818515No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT6390.12466127833410394No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC5440.10612791144562213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT226450.0141.092121
GGTATCA228850.0139.625128
GTATCAA229950.0139.082469
GTGGTAT230800.0138.414266
CAGTGGT231400.0138.11764
AGCAGTG231250.0138.007752
TGGTATC231650.0137.999627
AGTGGTA232200.0137.610735
GCAGTGG233150.0136.913993
CAACGCA229250.028.5169910-14
ATCAACG229350.028.47316410-14
TCAACGC229600.028.45470410-14
AGAGTAC229350.028.4480515-19
CAGAGTA229300.028.43541515-19
TACATGG228500.028.4089320-24
GTACATG228550.028.39641820-24
CGCAGAG230150.028.33039515-19
GCAGAGT230950.028.20108215-19
AGTACAT230400.028.19340520-24
AACGCAG233700.027.97398610-14
Produced by FastQC (version 0.11.5)