FastQCFastQC Report
Sun 19 Mar 2023
SRR4895934_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895934_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575160
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCT576950.0142.100971
GCACCTC577400.0141.990222
CACCTCG194500.0141.043273
ACCTCGT57600.0140.689624
ACCTCGA47000.0140.119374
CACCTCT161400.0139.542863
ACCTCGG69550.0139.163944
CACCTCA138200.0138.626193
ACCTCAT43250.0137.60194
ACCTCAG44400.0137.248374
ACCTCTA44900.0136.831154
CCTCGTA15650.0136.625265
ACCTCTG54350.0135.595434
ACCTCGC22650.0135.308274
CCTCGGA17100.0134.626465
CCTCGAT16150.0133.719335
CCTCGTG21150.0132.099065
CTCGTGA5300.0131.787526
ACCTCAA40450.0131.268894
CCTCGGT22950.0131.055075
Produced by FastQC (version 0.11.5)