FastQCFastQC Report
Sun 19 Mar 2023
SRR4895934_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895934_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575160
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG79371.379963836149941No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC57480.9993740872105152No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA27780.4829960358856666No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA18410.3200848459559079No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16430.2856596425342513No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC15660.2722720634258294No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCCAGGCGCAGTGGCTC13400.232978649419292No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT12310.21402740107100632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG11150.19385910007650045No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA11090.192815912094026No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC10600.18429654357048475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA8800.15300090409625147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC7790.1354405730579317No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCAAAAAAAAAAAAAAA7000.12170526462201821No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC6500.11301203143473121No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC6290.10936087349607065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144550.0139.157878
GTGGTAT144750.0138.816396
AAGCAGT144600.0138.709881
TGGTATC145300.0138.439587
GTATCAA145450.0138.29689
CAGTGGT147600.0136.135994
AGCAGTG148000.0135.523322
AGTGGTA149750.0134.08535
GCAGTGG151300.0132.47223
CGATAGG200.006282256528.80281635-39
CAACGCA143600.028.5571610-14
ATCAACG143550.028.53701410-14
CAGAGTA142600.028.45449815-19
AGAGTAC142800.028.42472815-19
TACATGG142100.028.3924920-24
TCAACGC144550.028.3794410-14
GTACATG142400.028.36301220-24
AGTACAT143450.028.12529220-24
CGCAGAG145400.028.09469815-19
GCAGAGT145900.027.80103915-19
Produced by FastQC (version 0.11.5)