FastQCFastQC Report
Sun 19 Mar 2023
SRR4895933_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895933_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151104
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAC2155000.0142.276982
ATGTACG763550.0142.114913
AGATGTA2158200.0142.066021
ATGTACC371300.0141.344913
TGTACGT236200.0140.905624
TGTACGA167500.0140.492654
TGTACGG284750.0140.232364
TGTACCG111050.0140.032074
ATGTACA470000.0139.98753
ATGTACT570250.0139.972933
TGTACTG210750.0139.220964
GTACGGT83750.0138.875825
TGTACTA133600.0138.640824
GTACGTG85050.0138.093815
GTACGTA54500.0138.088885
GTACGAT50600.0137.746225
GTACGAG62000.0137.592185
TGTACGC85650.0137.376074
GTACTAG49650.0136.649815
GTACGGA54000.0136.595985
Produced by FastQC (version 0.11.5)