FastQCFastQC Report
Sun 19 Mar 2023
SRR4895933_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895933_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151104
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG222151.0327255214066824No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC157160.7306015887655827No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC65730.30556402665793936No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA57620.26786245574365536No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56880.2644223617268156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG42350.1968756508285978No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG41580.1932960935408051No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT36410.16926192317991134No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC23900.11110573919252625No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC23390.1087348635863259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT553900.0135.143951
GGTATCA557800.0134.316518
GTGGTAT558150.0134.11626
GTATCAA561550.0133.522139
TGGTATC561100.0133.513727
CAGTGGT567800.0131.862184
AGCAGTG569200.0131.359472
AGTGGTA571650.0130.961525
GCAGTGG576950.0129.719773
TCAACGC557900.027.52439910-14
TACATGG554150.027.52382320-24
GTACATG554200.027.51094620-24
CAACGCA558900.027.48030710-14
ATCAACG559050.027.41368910-14
AGAGTAC557750.027.37689615-19
CAGAGTA559950.027.2924815-19
CGCAGAG563700.027.128815-19
AGTACAT563450.027.04652820-24
GCAGAGT568250.026.87103315-19
GAGTACA556250.026.80379320-24
Produced by FastQC (version 0.11.5)