FastQCFastQC Report
Sun 19 Mar 2023
SRR4895932_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895932_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738232
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATTC1743700.0142.106672
AGATTCG557050.0142.006123
ACAGATT1746850.0141.850421
GATTCGA135950.0141.665024
AGATTCA407050.0141.120423
GATTCGT186850.0140.534524
AGATTCC277100.0140.344163
GATTCGG180050.0140.022694
AGATTCT516850.0139.991233
ATTCGAG42500.0138.535195
GATTCTA133000.0137.947364
GATTCTG174050.0137.764864
GATTCCG69350.0137.725654
GATTCGC61150.0136.96134
GATTCAT134350.0136.773444
GATTCAA114950.0136.603564
ATTCGAT48950.0136.00765
GATTCAG136800.0135.678624
ATTCGTA54100.0135.577335
ATTCGTG59750.0135.283285
Produced by FastQC (version 0.11.5)