FastQCFastQC Report
Sun 19 Mar 2023
SRR4895932_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895932_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738232
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG325531.8727649703837002No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC202691.1660698917060552No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC73510.4229009706414334No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48420.27855890352956336No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG45980.264521651885364No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT39340.22632191790278858No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC33210.19105619963273027No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA33140.19065349159375733No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC26420.15199351985235574No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC26280.15118810377440986No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC23660.1361153171728515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT494200.0139.261661
GGTATCA495150.0139.255848
GTGGTAT497450.0138.452776
TGGTATC498750.0138.207387
GTATCAA498800.0138.193519
CAGTGGT503850.0136.765564
AGCAGTG506450.0135.921632
GCAGTGG511000.0134.866413
AGTGGTA511500.0134.72015
TCAACGC492700.028.5569710-14
CAACGCA493600.028.53407310-14
ATCAACG494950.028.4126110-14
AGAGTAC493650.028.40122615-19
TACATGG491600.028.35773520-24
CAGAGTA495250.028.31528915-19
GTACATG493350.028.30092620-24
CGCAGAG497950.028.18489315-19
AGTACAT496650.028.1099820-24
GCAGAGT504250.027.73565715-19
GAGTACA494300.027.7075920-24
Produced by FastQC (version 0.11.5)