FastQCFastQC Report
Sun 19 Mar 2023
SRR4895931_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895931_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408911
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC1413450.0142.103791
CACGACC1415000.0141.948122
ACGACCT367350.0141.708923
ACGACCG462650.0141.669083
CGACCGT137000.0140.897754
ACGACCA326850.0140.730773
CGACCTA98950.0140.113434
CGACCAT100550.0139.939734
CGACCTG122550.0139.887124
CGACCGA118900.0139.625124
ACGACCC267750.0139.155063
GACCGTA40150.0138.479345
CGACCGG156550.0138.328174
CGACCTT115400.0137.99184
GACCGAT37300.0137.976235
CGACCAG108250.0137.360664
GACCGTG44600.0135.850115
CGACCAA92700.0135.48844
GACCTAG33700.0135.15965
GACCGTT44500.0134.393395
Produced by FastQC (version 0.11.5)