FastQCFastQC Report
Sun 19 Mar 2023
SRR4895931_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895931_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408911
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG178911.2698460016282078No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC124470.8834482802675258No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC75910.5387849197003927No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG49480.35119322654163393No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37410.2655242240283453No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA34220.24288262352980422No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCTTGAGTCTCGGTGCGA26990.19156639418671584No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC16020.1137048401212No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT15040.10674911332227514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC14590.10355515713909537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT440700.0139.9151
GGTATCA442200.0139.51628
GTGGTAT442650.0139.341846
TGGTATC445050.0138.655127
GTATCAA449100.0137.420759
CAGTGGT451450.0136.625694
AGCAGTG451050.0136.592652
AGTGGTA454950.0135.590425
GCAGTGG455950.0135.23533
CAACGCA440800.028.58312610-14
TCAACGC441100.028.5538910-14
ATCAACG442550.028.44731910-14
GTACATG440300.028.38112420-24
AGAGTAC442800.028.35646615-19
TACATGG440650.028.316120-24
CAGAGTA443800.028.30879215-19
AGTACAT442450.028.24321220-24
CGCAGAG447000.028.12868715-19
GCAGAGT450400.027.84281315-19
GAGTACA441550.027.76920320-24
Produced by FastQC (version 0.11.5)