FastQCFastQC Report
Sun 19 Mar 2023
SRR4895930_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895930_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412306
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTGC1416500.0142.114042
AATGTTG1420600.0141.70391
TGTTGCG480850.0140.180073
TGTTGCA306850.0140.097983
GTTGCGA108450.0139.316644
GTTGCTA94200.0138.299934
GTTGCGT147400.0137.701644
TGTTGCT398700.0137.388633
GTTGCAT96400.0137.213734
GTTGCAG107650.0136.379734
TGTTGCC265550.0136.068713
GTTGCGG178300.0136.021414
TTGCGTA38800.0135.924165
GTTGCTG142300.0135.725364
GTTGCAA81950.0135.318534
GTTGCCG71950.0135.206794
TTGCGAG38350.0133.802355
TTGCGAT35350.0133.061115
GTTGCGC58800.0131.749454
GTTGCCT59400.0131.618474
Produced by FastQC (version 0.11.5)