FastQCFastQC Report
Sun 19 Mar 2023
SRR4895930_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895930_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412306
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG215671.5270769932295127No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC124860.8840860266826027No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA77850.5512261507067165No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT44460.31480429878510746No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40570.2872606927960371No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC30500.21595886443872644No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA29310.20753292841636303No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA28850.20427584390351666No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC27390.19393814088448255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT25260.17885642346630262No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT25110.17779433069037448No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC24210.1714217740348055No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC23760.168235495707021No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC17900.12674307126076076No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG15630.11067006725171456No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC15420.10918313736541514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTAAGCTGCAGCAAGAG14250.10089881371317547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT405000.0138.157941
GGTATCA406100.0137.704368
GTATCAA406700.0137.53669
GTGGTAT406500.0137.480296
TGGTATC409150.0136.695457
CAGTGGT412800.0135.469334
AGTGGTA414050.0135.025575
AGCAGTG415600.0134.58222
GCAGTGG418400.0133.647133
TCAACGC404450.028.26244710-14
CAACGCA405100.028.24553710-14
AGAGTAC405000.028.0753315-19
ATCAACG406900.028.05334910-14
GTACATG404000.028.04442220-24
TACATGG403500.028.03991720-24
CAGAGTA406500.027.97881515-19
CGCAGAG408500.027.8947115-19
AGTACAT407100.027.8167220-24
AACGCAG413000.027.70873510-14
GCAGAGT410250.027.66339715-19
Produced by FastQC (version 0.11.5)