FastQCFastQC Report
Sun 19 Mar 2023
SRR4895929_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895929_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718805
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTC719500.0142.401872
AATCCGT720000.0142.3031
TCCGTCG231100.0141.461183
CCGTCTA53400.0140.805514
TCCGTCT201250.0140.238663
CCGTCGA57650.0140.191674
CCGTCGT76500.0139.559314
CCGTCTG62550.0138.780494
CGTCTAG16850.0138.733835
CGTCGAT19250.0138.467975
TCCGTCA173350.0138.264743
CCGTCAA45300.0137.820664
CGTCAAG15050.0137.804985
TCCGTCC127800.0137.521513
CGTCGGA16700.0137.419345
CGTCGAA15900.0137.161675
CCGTCGG73800.0137.13254
CGTCGTA20600.0137.0055
CCGTCAG56600.0136.748264
CGTCTAA15000.0136.36385
Produced by FastQC (version 0.11.5)