FastQCFastQC Report
Sun 19 Mar 2023
SRR4895929_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895929_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718805
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG98001.3633739331251176No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC70760.9844116276319725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA32250.4486613198294392No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC23350.3248447075354234No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22270.3098197703132282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC16410.22829557390390995No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG13750.19128971000479963No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT11960.1663872677569021No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA11560.1608224761931261No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC10880.1513623305347069No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA10400.14468458065817574No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC10040.13967626825077734No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT9750.13564179436703974No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTCTAAGCTGCAGCAAGA9540.13272027879605736No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTAAGCTGCAGCAAGAG8490.11811270094114537No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAGCAAAAAAAAAAAAAAA7370.10253128456257261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT200700.0139.75951
GGTATCA202050.0138.903638
GTATCAA203200.0138.294689
GTGGTAT203350.0138.086446
TGGTATC204600.0137.2787
CAGTGGT204900.0136.971594
AGCAGTG205400.0136.351142
AGTGGTA206100.0136.174095
GCAGTGG206700.0135.667793
CGCACGA200.00628559728.79987560-64
CAACGCA201050.028.5133810-14
AGAGTAC199350.028.48926715-19
ATCAACG201200.028.44202410-14
CAGAGTA199750.028.43221715-19
TCAACGC201600.028.42130510-14
GTACATG199100.028.3876220-24
TACATGG199350.028.27256420-24
AGTACAT200100.028.21696920-24
CGCAGAG203300.028.12697815-19
GCAGAGT201750.028.10039715-19
Produced by FastQC (version 0.11.5)