FastQCFastQC Report
Sun 19 Mar 2023
SRR4895928_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895928_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559395
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG59721.0675819412043368No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC43480.7772682987870825No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA29710.5311095022300878No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC23570.4213480635329239No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18270.3266028477194111No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG17020.3042572779520732No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT14000.2502703813941848No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA12110.21648387990596982No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA9590.17143521125501657No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC8050.14390546930165624No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTAAGCTGCAGCAAGAG6790.12138113497617964No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC6320.11297920074366057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT164600.0139.08941
GGTATCA165300.0138.668648
GTGGTAT165300.0138.537986
TGGTATC166150.0137.959237
GTATCAA166800.0137.507939
CAGTGGT166700.0137.37454
AGCAGTG167450.0136.67912
AGTGGTA169950.0134.832155
GCAGTGG170350.0134.521413
ACGCAGT700.001091572141.1524121
CAACGCA164900.028.438410-14
ATCAACG164900.028.38601110-14
GTACATG163550.028.3738220-24
TCAACGC165350.028.33487910-14
TACATGG163800.028.31293320-24
AGAGTAC164650.028.28919415-19
CGCAGAG166100.028.14625715-19
AGTACAT164900.028.1415320-24
CAGAGTA165650.028.12711115-19
GCAGAGT167500.027.77347215-19
Produced by FastQC (version 0.11.5)