FastQCFastQC Report
Sun 19 Mar 2023
SRR4895927_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895927_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11395947
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGATC11419950.0142.244522
AAGAGAT11454600.0141.814241
GAGATCA2827100.0141.69013
GAGATCG3327250.0141.68793
GAGATCT3607700.0141.377173
GAGATCC1717200.0141.146013
AGATCGA864200.0140.84584
AGATCGT1148450.0140.484344
AGATCTG1104800.0140.286824
AGATCTA943050.0139.975114
AGATCAT951400.0139.772934
AGATCAG883950.0139.400184
AGATCCG410000.0138.807274
GATCGAT307250.0138.206915
AGATCAA794100.0138.182754
AGATCGC379900.0137.516824
GATCTAG285800.0137.208045
GATCGAG276550.0137.081055
AGATCGG993700.0136.993274
AGATCTT1233000.0136.642934
Produced by FastQC (version 0.11.5)