FastQCFastQC Report
Sun 19 Mar 2023
SRR4895927_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895927_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11395947
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC1513951.328498632013645No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG766110.6722653238032785No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA617880.54219276379576No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTACTGAGAACTCATAAG583730.5122259694608969No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC451050.3957986115590043No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA373020.32732689964247813No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG298130.26161055329583405No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG269190.23621555979507453No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTTACTGAGAACTCATAA265760.2332057177872098No Hit
AGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTG235360.20652956704695097No Hit
CACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCT233370.20478333217941433No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT224310.19683313725485035No Hit
GTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAG215580.18917251896661155No Hit
GTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGT207790.18233675533942023No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT201090.1764574721170606No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAGTTACTGAGAACTCAT199030.17464981190242462No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC191450.16799832431653114No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT190810.167436721143052No Hit
CACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTT187620.16463748032524195No Hit
AAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAA186840.16395302645756427No Hit
TGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGA186300.16347917377994123No Hit
TTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATG185610.16287369535853405No Hit
AAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGA185080.16240861773049664No Hit
GGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACC184450.1618557896066031No Hit
TGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTC184270.1616978387140621No Hit
GTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTG181090.15890737294583768No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT180670.158538820863242No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT180100.15803864303686213No Hit
AGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTT179910.15787191709473553No Hit
CAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTT177310.15559040420247655No Hit
ACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATG176970.15529205251656578No Hit
ACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGT174090.15276483823590967No Hit
TATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCT173890.15258933724419743No Hit
AAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTG172540.1514047055501399No Hit
GAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTC172340.15122920455842764No Hit
CTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGA172010.1509396279221025No Hit
CTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTT171450.15044822514530826No Hit
CTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAAT170870.14993927226934275No Hit
AGTAATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTA169330.14858791463315862No Hit
AATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTC167530.14700840570774856No Hit
GGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACT165160.14492871895595863No Hit
ATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCC164960.14475321796424642No Hit
GGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCT164520.1443671157824795No Hit
ATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATT164320.14419161479076728No Hit
GTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAAT162150.14228742903068958No Hit
CAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCT161990.1421470282373198No Hit
GTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGA160860.14115544763414573No Hit
ATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTG160660.1409799466424335No Hit
GTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTT160460.14080444565072125No Hit
TTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATAC159870.1402867177251702No Hit
AATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTT159390.13986551534506084No Hit
TGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACT156790.13758400245280186No Hit
TGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTAT154880.1359079679819501No Hit
GTCACCTCAGAGTCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCAC152240.13359135489134866No Hit
CTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAA151690.13310872716414002No Hit
CACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTT150660.13220489705682203No Hit
ACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTC150610.132161021808894No Hit
AAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATA150330.13191532042049686No Hit
TATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGAT148640.13043233704052853No Hit
TGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAAC147970.1298444087182926No Hit
ATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTG146330.1284053005862523No Hit
TACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAG145390.1275804459252048No Hit
TGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTG144960.1272031187930235No Hit
GCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTG141070.12378962450422067No Hit
CGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAA140010.12285946924814586No Hit
TGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCAT139720.12260499281016313No Hit
ATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGG139710.12259621776057751No Hit
TACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGAT138220.1212887353723214No Hit
TTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGC138100.12118343477729406No Hit
ATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACA138090.12117465972770847No Hit
CAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACA137470.12063060665340054No Hit
ACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTG137120.12032347991790414No Hit
CTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGC137060.12027082962039047No Hit
GTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATA136290.11959515080229839No Hit
GCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAG136230.11954250050478472No Hit
CTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGT135810.11917394842218905No Hit
TGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCC135730.11910374802550415No Hit
ATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAA135550.11894579713296315No Hit
TGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTA135210.11864744544705236No Hit
CTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCT134770.11826134326528547No Hit
AATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGG133660.1172873127612826No Hit
AATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAA133630.11726098761252575No Hit
TGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAA131650.11552352779457468No Hit
GATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATG131540.11542700224913295No Hit
ATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATA131340.11525150125742073No Hit
AGAGTCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCACAGTAATGG130350.11438277134844518No Hit
CATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCC130330.11436522124927398No Hit
AGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAAT128830.11304896381143226No Hit
ATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGA128780.11300508856350419No Hit
AGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCAC128420.1126891867784222No Hit
ACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTT128370.11264531153049413No Hit
GGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTT128130.11243471034043946No Hit
AGGGTCACAGTAATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCA127700.11205738320825816No Hit
ATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGA127330.11173270637359055No Hit
ATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCT126970.11141680458850853No Hit
TAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGG126640.11112722795218336No Hit
CCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGC126430.11094295191088552No Hit
CCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTG126350.11087275151420062No Hit
GTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTT125970.1105392996299474No Hit
TTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGA125310.10996014635729703No Hit
GGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACG124610.10934589288630422No Hit
GAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTT122790.10774883386172295No Hit
GTGAACCCCGTGGTCTCCTACGCCGTGGCTCTCAGCTGTCAATGTGCACT122200.10723110593617187No Hit
TCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAA121610.1067133780106208No Hit
AACAGGGTCACAGTAATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTG121510.10662562751476469No Hit
AAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTA120470.10571302235786108No Hit
CTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGT120340.10559894671324814No Hit
AAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATAC119880.10519529443231No Hit
GGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTT119350.10473021680427262No Hit
ATATAACAGGGTCACAGTAATGGGGGGTTTCAAAGTGGAGAACCACACGG119270.10466001640758772No Hit
AGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTAC119210.10460736611007405No Hit
TATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGAC119150.10455471581256037No Hit
TCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCA118750.10420371382913593No Hit
GTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACT117700.10328233362264672No Hit
TGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTT115720.10154487380469567No Hit
TTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGAC115590.1014307981600827No Hit
GATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAG115550.10139569796174025No Hit
AGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGA114740.10068491894530573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1758250.0139.550118
AAGCAGT1760300.0139.253271
TGGTATC1764000.0139.095237
GTGGTAT1763550.0139.044986
GTATCAA1768750.0138.790889
CAGTGGT1788600.0137.157994
AGCAGTG1794600.0136.515492
AGTGGTA1804450.0135.925285
GCAGTGG1822650.0134.505433
TCAACGC1751650.028.613810-14
ATCAACG1756150.028.51998110-14
CAACGCA1760300.028.49282510-14
AGAGTAC1754650.028.43297615-19
TACATGG1749350.028.37997820-24
GTACATG1752650.028.37008920-24
CGCAGAG1772550.028.2262715-19
CAGAGTA1769450.028.21143315-19
AGTACAT1764050.028.18266920-24
GCAGAGT1786350.027.92518415-19
GAGTACA1752250.027.8473320-24
Produced by FastQC (version 0.11.5)