FastQCFastQC Report
Sun 19 Mar 2023
SRR4895926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3449637
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC273990.7942574827438366No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG260450.7550069760963255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA192800.5588993856455042No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTACTGAGAACTCATAAG190680.5527538114879913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC173980.5043429207189046No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG85960.24918563895273618No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTTACTGAGAACTCATAA83860.2430980419099169No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG81890.23738729611260545No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78030.2261977129767567No Hit
AGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTG70850.2053839288017841No Hit
CACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCT68300.19799184667836064No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT65490.18984606206392152No Hit
GTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAG63660.18454115606946472No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAGTTACTGAGAACTCAT60430.1751778520464617No Hit
GTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGT58650.17001788883873867No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT57540.16680015897324849No Hit
AGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTT55020.15949504252186533No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT54420.15775572908105984No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC53410.15482788478903722No Hit
CACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTT53280.15445103354352938No Hit
AAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGA52860.1532335141349655No Hit
AAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAA52800.15305958279088497No Hit
TGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGA52640.1525957658733368No Hit
TGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTC52220.15137824646477296No Hit
GTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTG52110.1510593723339586No Hit
GGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACC52000.15074049820314428No Hit
AGTAATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTA51750.15001578426947532No Hit
TTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATG51520.14934904745049984No Hit
CAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTT51270.1486243335168309No Hit
ACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGT51130.14821849371397627No Hit
TATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCT50840.14737782555092027No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT50000.14494278673379257No Hit
ACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATG49820.14442099270155093No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT49670.14398616434134953No Hit
GAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTC48730.14126123995075424No Hit
CTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTT48550.1407394459185126No Hit
CTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGA48100.13943496083790846No Hit
ATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCC47200.1368259906767002No Hit
AAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTG47080.1364781279885391No Hit
CTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAAT46600.13508667723589468No Hit
GGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACT46570.1349997115638544No Hit
GGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCT46530.13488375733446736No Hit
GTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAAT46460.13468083743304005No Hit
ATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATT46290.13418803195814516No Hit
ATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTG46100.13363724936855675No Hit
CAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCT46020.13340534090978268No Hit
GTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGA45980.13328938668039564No Hit
AATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTC45810.13279658120550075No Hit
TTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATAC45510.13192692448509802No Hit
AATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTT45230.13111524487938875No Hit
TGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACT45200.13102827920734847No Hit
TGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTAT44360.12859324039022077No Hit
GTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTT43950.12740470953900368No Hit
GTCACCTCAGAGTCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCAC43940.12737572098165692No Hit
CACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTT43440.125926293114319No Hit
ACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTC43210.12525955629534355No Hit
CTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAA42800.12407102544412645No Hit
ATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTG42600.12349125429719128No Hit
CGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAA42540.12331732295311072No Hit
TATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGAT42390.12288249459290934No Hit
ATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGG42050.12189688364311956No Hit
TACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAG41830.12125913538149086No Hit
TGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTG41500.12030251298904783No Hit
TGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAAC41430.12009959308762051No Hit
ACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTG40330.11691085177947709No Hit
TGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCAT40100.11624411496050165No Hit
TGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTA40050.11609917217376783No Hit
TTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGC39920.11572232092825999No Hit
ATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACA39570.11470772142112345No Hit
GCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTG39500.11450480151969614No Hit
AAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATA39220.1136931219139869No Hit
CTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGT39170.11354817912725311No Hit
TACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGAT39010.11308436220970497No Hit
GTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTT38840.11259155673481007No Hit
TGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCC38610.11192481991583463No Hit
ATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAA38610.11192481991583463No Hit
CAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACA38500.11160594578502028No Hit
GTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATA38490.11157695722767352No Hit
AATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAA38400.11131606021155269No Hit
GCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAG38350.1111711174248189No Hit
AGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAAT38270.11093920896604484No Hit
AGAGTCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCACAGTAATGG38110.11047539204849668No Hit
CTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGC38100.11044640349114994No Hit
AGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCAC38010.11018550647502912No Hit
AATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGG37470.10862012437830415No Hit
CCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGC37300.10812731890340926No Hit
GGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTT37210.10786642188728843No Hit
ACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTT37190.10780844477259492No Hit
TGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAA37140.10766350198586111No Hit
GATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATG37110.10757653631382086No Hit
AGGGTCACAGTAATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCA36810.1067068795934181No Hit
CTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCT36650.10624306267586994No Hit
ATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGA36470.10572126864362831No Hit
ATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATA36400.10551834874220099No Hit
GGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACG35960.10424285221894362No Hit
TAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGG35500.10290937858099274No Hit
ATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGA35320.10238758454875108No Hit
TCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAA35250.10218466464732376No Hit
GAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTT34930.10125703081222748No Hit
TTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGA34880.1011120880254937No Hit
ATATAACAGGGTCACAGTAATGGGGGGTTTCAAAGTGGAGAACCACACGG34850.10102512235345341No Hit
CATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCC34790.10085119100937288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT515600.0139.594221
GGTATCA516300.0139.476098
GTGGTAT518200.0138.797976
TGGTATC519150.0138.654947
GTATCAA521300.0138.179769
AGCAGTG525050.0136.930882
CAGTGGT527850.0136.396914
AGTGGTA529000.0136.045965
GCAGTGG532100.0135.211363
TCAACGC515700.028.58104910-14
CAACGCA516150.028.55892210-14
ATCAACG517650.028.45669610-14
TACATGG515000.028.42459320-24
AGAGTAC518050.028.33743915-19
GTACATG518400.028.27705420-24
CGCAGAG519950.028.27266115-19
AGTACAT520050.028.16518620-24
CAGAGTA522250.028.13987515-19
GCAGAGT526000.027.92830815-19
AACGCAG528450.027.88329710-14
Produced by FastQC (version 0.11.5)