FastQCFastQC Report
Sun 19 Mar 2023
SRR4895925_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895925_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025041
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA1027600.0142.18262
TTCACGC1028550.0142.051281
CACGCAT319400.0141.338293
ACGCATG109450.0140.980444
ACGCATA80500.0140.861084
ACGCAAT78350.0140.723954
CACGCAG325750.0140.683533
CACGCAA244950.0140.361043
ACGCAGT98200.0140.220764
ACGCATT87550.0139.124184
CGCATGA28900.0138.844045
CGCATAG27100.0138.335365
CACGCAC150300.0138.085833
ACGCAGA81600.0137.914114
ACGCAGG112900.0137.808654
CGCATGT35050.0137.45975
ACGCAAG84000.0137.452444
CGCAATG25250.0137.180255
CGCAATA21900.0137.010775
CGCATAT27300.0136.53875
Produced by FastQC (version 0.11.5)