FastQCFastQC Report
Sun 19 Mar 2023
SRR4895925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025041
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG104361.018105617238725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC68450.6677781669221036No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC44430.4334460767910747No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC30330.29589060340025425No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG25070.2445755828303453No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22250.21706448815218124No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC19410.1893582793273635No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT14370.1401895143706447No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA12630.12321458361177749No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC11980.11687337384553398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT279850.0139.581381
GGTATCA280750.0139.356458
GTGGTAT282450.0138.339266
GTATCAA283250.0138.228159
TGGTATC284250.0137.564547
CAGTGGT286950.0136.219994
AGCAGTG287200.0135.9342
AGTGGTA287700.0135.814825
GCAGTGG291000.0134.332173
CAACGCA278900.028.63959310-14
TCAACGC280550.028.49168610-14
ATCAACG281350.028.35437410-14
GTACATG280050.028.26057820-24
AGAGTAC282400.028.17246215-19
TACATGG281200.028.1296420-24
CGCAGAG283250.028.11333815-19
CAGAGTA283150.028.11309415-19
AGTACAT281850.028.08520520-24
GCAGAGT285300.027.89618915-19
GAGTACA279800.027.80719620-24
Produced by FastQC (version 0.11.5)