FastQCFastQC Report
Sun 19 Mar 2023
SRR4895924_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895924_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714015
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTA717450.0141.870512
TGGTAGC147050.0141.441164
GTATCCG31800.0141.211156
TGGTATC123700.0141.17314
GGTAGCC122450.0141.100775
GGTATCC95550.0141.064075
GTGGTAA169000.0140.868963
GTGGTAG238150.0140.850023
GTAGCCG39850.0140.767366
GTGGTAT223500.0140.73823
TGGTACC69000.0140.489994
TGGTAAC91300.0140.448144
GTGGTAC92450.0140.397253
TGGTGGT726550.0140.09361
TAGCCTA8450.0140.025447
GTAGCCT35400.0139.133076
GGTACCC61700.0138.97485
GGTATGC16550.0138.679875
GTAACCG25200.0138.030436
GGTAACC74800.0137.791445
Produced by FastQC (version 0.11.5)