FastQCFastQC Report
Sun 19 Mar 2023
SRR4895924_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895924_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714015
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG106771.4953467364131006No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC67410.9440978130711539No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA48870.6844394025335602No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC26360.36917991918937276No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT25630.35895604434080514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT21970.3076966170178498No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19740.27646478015167747No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC18690.2617592067393542No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA17250.2415915632024537No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC17230.241311457042219No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT14870.208258930134521No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA14030.19649447140466236No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA12360.1731056070250625No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG11570.1620414136957907No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA10770.15083716728640154No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCGAGATTCCCCCAGTAG9990.13991302703724712No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT9540.13361063843196572No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTAATGAGGCGAACC9090.12730824982668432No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGATTCCCCCAGTAGCGGC8830.12366686974363283No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCGAACGGGGAGCAGCCC8340.11680426881788197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC7840.10980161481201375No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC7530.10545996932837544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC7290.10209869540555871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT218300.0140.311511
GGTATCA219350.0139.633568
GTATCAA221150.0138.562169
GTGGTAT221400.0138.340656
CAGTGGT222450.0137.784764
TGGTATC222950.0137.443477
AGCAGTG223100.0137.195852
GCAGTGG224700.0136.475363
AGTGGTA225200.0136.102235
CAACGCA219050.028.61576710-14
ATCAACG219250.028.5699610-14
TCAACGC219750.028.52461210-14
GTACATG217850.028.47594320-24
AGAGTAC218750.028.45752515-19
TACATGG217850.028.40323320-24
CAGAGTA220000.028.30892615-19
AGTACAT219400.028.30758320-24
CGCAGAG221100.028.27229115-19
GCAGAGT221250.028.12946315-19
GAGTACA219000.027.75435620-24
Produced by FastQC (version 0.11.5)