FastQCFastQC Report
Sun 19 Mar 2023
SRR4895923_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895923_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365467
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTCA1372550.0141.813262
TGGCTTC1375650.0141.49371
GCTTCAG453300.0141.088363
GCTTCAA331800.0140.617323
GCTTCAT431800.0138.506563
CTTCATG141750.0137.472144
GCTTCAC176350.0137.377293
CTTCAGG157550.0137.121874
CTTCAGT141300.0136.094014
CTTCAAT118000.0134.094274
CTTCACG50150.0133.881074
CTTCAGA116550.0133.13294
CTTCAAG115700.0133.00214
TTCATCG20150.0127.694265
TTCATGG57900.0127.039975
CTTCATA126100.0126.9503564
TTCAGTA39500.0126.310715
CTTCAGC60400.0125.911894
TTCATAG44750.0124.552645
TTCAGTG52550.0124.104525
Produced by FastQC (version 0.11.5)