FastQCFastQC Report
Sun 19 Mar 2023
SRR4895923_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895923_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365467
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG130840.9582069724131013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC78030.5714528436058872No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC51400.3764279913026093No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT50370.3688847844730045No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC37780.27668189710919416No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC32870.24072350338748574No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG29360.21501801215261887No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28900.2116492013355138No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT26670.19531779237433053No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC25730.18843370070459411No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT22310.1633873246295956No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA19930.14595739040196504No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC19630.14376033986907044No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC18790.13760859837696554No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGCCCCTCATTTACATAAA18330.13423978755986046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT418600.0139.193191
GGTATCA418850.0139.027988
GTGGTAT421050.0138.198966
TGGTATC423900.0137.303777
GTATCAA424800.0137.012869
AGTGGTA425700.0136.740135
AGCAGTG426850.0136.45232
CAGTGGT427700.0136.151184
GCAGTGG429900.0135.400473
CAACGCA416950.028.61610610-14
TCAACGC418200.028.51679610-14
GTACATG418700.028.328420-24
ATCAACG420800.028.30980710-14
TACATGG419550.028.24355120-24
CAGAGTA422550.028.16188615-19
AGAGTAC422800.028.14523315-19
CGCAGAG423300.028.1119915-19
AGTACAT421650.028.09605420-24
AACGCAG425600.028.04465310-14
GCAGAGT426050.027.886615-19
Produced by FastQC (version 0.11.5)