FastQCFastQC Report
Sun 19 Mar 2023
SRR4895922_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895922_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1665257
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG186391.1192866926846727No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC133900.8040800909409179No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC96700.5806911485734635No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG66520.3994578614592222No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45790.27497257180122947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC34800.20897675253729606No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC24930.14970662186076983No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC23100.1387173271152741No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA22800.1369158033865043No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTTCTTGCTGCAGCAACG19230.11547767101414376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA410950.0139.038918
GTGGTAT412750.0138.36286
TGGTATC414550.0137.814127
AAGCAGT414550.0137.698331
GTATCAA417300.0136.888699
CAGTGGT422250.0135.283954
AGTGGTA422400.0135.201815
AGCAGTG424450.0134.43572
GCAGTGG428700.0133.153343
CAACGCA409200.028.5777210-14
TCAACGC409650.028.53929710-14
ATCAACG411600.028.35511210-14
GTACATG410100.028.32341620-24
TACATGG410000.028.27412420-24
AGAGTAC412300.028.24410615-19
CAGAGTA413850.028.15919915-19
CGCAGAG415400.028.13038815-19
AGTACAT413350.028.09375220-24
AACGCAG422450.027.6813910-14
GAGTACA411500.027.65313520-24
Produced by FastQC (version 0.11.5)