FastQCFastQC Report
Sun 19 Mar 2023
SRR4895921_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895921_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329498
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGAG1336650.0141.781131
GAAGAGA1343000.0141.110762
AAGAGAT419700.0139.509163
AAGAGAG437850.0137.958513
AAGAGAC181000.0136.366613
AAGAGAA350250.0130.970063
AGAGATC63400.0127.821934
AGAGACC65700.0127.46964
AGAGAGT134850.0126.745454
AGAGATA127150.0126.6852954
AGAGATG151850.0125.604564
AGAGAGC72250.0122.720374
AGAGACG54100.0122.6553654
AGAGACT42200.0122.281364
AGAGATT125050.0122.087134
GAGATAG44300.0121.9555
GAGATCG21050.0121.8946155
AGAGAAT117350.0121.777044
GAGACGT16250.0120.179755
AGAGAGA120150.0119.532344
Produced by FastQC (version 0.11.5)